Exhibit 99.1

2021 Reference Form Itaú Unibanco Holding S.A.

Itaú Unibanco Holding S.A. REFERENCE FORM Base Date: 12.31.2021 (in accordance with Attachment 24 to CVM lnstruction No. 480 of December 7, 2009 “CVM lnstruction No. 480”, as amended) Identification Head Office ltaú Unibanco Holding S.A., a corporation enrolled under the National Register of Legal Entities/Ministry of Finance (CNPJ/MF) under No. 60.872.504/0001-23, with its Articles of lncorporation registered with the Trade Board of the State of São Paulo under NlRE No. 35.3.0001023-0, and registered as a publicly-held company with the Brazilian Securities and Exchange Commission ("CVM") under No. 19348 ("Bank" or "lssuer"). The lssuer's head office is located at Praça Alfredo Egydio de SouzaAranha, 100, Torre Olavo Setubal, Parque Jabaquara, in the City and State of São Paulo, Brazil, Zip Code 04344-902. Investor Relations Office lndependent Auditors Firm The lnvestor Relations department is located at Praça Alfredo Egydio de Souza Aranha, 100, Piso Metrô, Torre Olavo Setubal, Parque Jabaquara, in the City and State of São Paulo, Brazil, Zip Code 04344-902. The Group Head of lnvestor Relations is Mr. Renato Lulia Jacob. The lnvestor Relations Department's telephone number is (0xx11) 2794-3547, fax number is +55 11 5019-8717, and email is ri@itau-unibanco.com.br. PricewaterhouseCoopers Auditores Independentes Ltda. for the years ended 12/31/2021, 12/31/2020 and 12/31/2019. Bookkeeping Agent Itaú Corretora de Valores S.A. Stockholders Service The lssuer's stockholders' service is carried out at the branches of ltaú Unibanco S.A., the head office of which is located at Praça AlfredoEgydio de Souza Aranha, 100, Torre Olavo Setubal, Parque Jabaquara, in the City and State of São Paulo, Brazil, Zip Code 04344-902. Newspapers from which the Company discloses lnformation O Estado de São Paulo newspaper. Website http://www.itau.com.br/relacoes-com-investidores The information included in theCompany's website is not an integral part of this Reference Form. Last update of this Reference Form 10/14/2022

Historical resubmission Version Reasons for resubmission Date of update V2 Updated items: 10.3 and 15.7 06/14/2022 Updated items: 10.3, 12.1, 12.2, 12.5/6, 12.7/8, 12.9, V3 07/11/2022 12.12, 13.1, 15.4 and 15.7 V4 Updated item: 7.2 07/20/2022 V5 Updated items: 11.1, 11.2 and 12.5/6 08/08/2022 V6 Updated items: 19.1 and 12.5/6 09/02/2022 V7 Updated items: 12.5/6, 12.7/8, 12.12 and 15.7 10/10/2022 V8 Updated items: 12.9, 15.1/2, 15.3, 15.4, 15.8 and 19.2 10/14/2022

12.12. OTHER SIGNIFICANT INFORMATION Addition Information of items 12.5/6, 12.7/8, 12.9 and 12.10 A—Total Number of Meetings Held by Body: Body Meetings Board of Directors (1) 12 Fiscal Council (1) 4 Audit Committee (2) 49 Disclosure and Trading Committee (3) 4 Strategy Committee (4) 6 Capital and Risk Management Committee (4) 12 Nomination and Corporate Governance Committee (4) 1 Related Parties Committee (4) 9 Personnel Committee (4) 5 Compensation Committee (4) 4 Environmental, Social and Climate Responsibility Committee (4) 2 (1) period from June 15, 2021 to February 25, 2022 (2) period from June 16, 2021 to February 25, 2022 (3) period from April 19, 2021 to January 28, 2022 (4) period from April 29, 2021 to February 25, 2022 B – Consecutive Terms of Office: For the number of consecutive terms of office: 1) of the members of the Board of Directors, Fiscal Council, Audit Committee, other committees and Executive Board, the following criteria were adopted: (a) counting as from ltaú Unibanco merger on November 3, 2008; (b) inclusion of terms of office with periods shorter than one year in case a member joins the body after the beginning of a term of office; and (c) inclusion of current terms of office. For the number of consecutive terms of office of the members of the Disclosure and Trading Committee, the following criteria were adopted: (a) counting as from ltaú Unibanco Merger on November 3, 2008; (b) inclusion of current terms of office. C—Meeting Attendance Percentage: For calculation of the meeting attendance percentage: (a) of the members of the Board of Directors and Fiscal Council, the meetings held from the moment members took office on June 15, 2021 until February 25, 2022 were considered; (b) of the members of the Audit Committee, meetings held from the moment the members took office on June 15, 2021 until February 25, 2022 were considered; (c) of the members of the other committees, meetings held from the moment the members took office on April 29, 2021 until February 25, 2022 were considered; (d) of the members of the Disclosure and Trading Committee, meetings held in the period from April 19, 2021 to February 28, 2022 were considered; (e) there is no calculation of the meeting attendance percentage of the management member Cesar Nivaldo Gon because the meetings do not involve her reelection to the Company’s Board of Directors; (f) there is no calculation of the meeting attendance percentage of the management members Eduardo Hiroyuki Miyaki and Gilberto Frussa because the meetings do not involve his reelection to the Company’s Fiscal Council; (g) there is no calculation of the meeting attendance percentage of the management members Reinaldo Guerreiro, João Costa and Rene Guimarães Andrich as alternate members were not required to attend the meetings of the Company’s Fiscal Council; (h) there is no calculation of the meeting attendance percentage of the management member Álvaro Felipe Rizzi Rodrigues because it was formalized that he became part of the Disclosure and Trading Committee on April 27, 2022; (i) there is no calculation of the meeting attendance percentage of members of the Executive Board, and the percentage is zero because the field to be filled in the Empresas-Net system is disabled.

D - Independence Criterion for the Members of the Board of Directors and of the Audit Committee: The members of the Board of Directors Cesar Nivaldo Gon, Fábio Colletti Barbosa, Frederico Trajano lnácio Rodrigues, Maria Helena dos Santos Fernandes de Santana and Pedro Luiz Bodin de Moraes are deemed independent. An independent member is a member who has no commercial or other relationship with the Company, with a company under the same control, with the controlling stockholder or with member of a management body that may (i) give rise to a conflict of interests; or (ii) impair its capacity and exemption from analysis and assessment. All members of the Audit Committee are deemed independent, in conformity with applicable regulation and under the terms and conditions of the Bylaws: a) not to be, or not to have been, in the past (12) twelve months: (i) an officer of the company, its controlling company or affiliates, controlled or jointly-controlled companies, directly or indirectly; (ii) an employee of the company, its controlling company or affiliates, controlled or jointly-controlled companies, directly or indirectly; (iii) a responsible technician, officer, manager, supervisor or any other member, with a managerial function, of the team involved in the audit work of the company; or (iv) a member of the Supervisory Council of the company, its controlling company or affiliates, controlled or jointly-controlled companies, directly or indirectly; b) not to be a spouse, a partner or family member in a direct or a collateral line or by affinity, up to twice removed, of the said persons in sections “a”, “(i)” and “(iii)”; and c) not to hold positions, in particular in advisory boards, boards of directors or supervisory councils in companies that may be deemed as competitors in the market or where a conflict of interests may arise. E – Type of Audit Committee lt is clarified that, pursuant to article 22, paragraph 2 from the Law nº 6,385/76, the Audit Committee adheres to the Resolution 4,910/21 of the National Monetary Council, which explains the reason why it is not compliant with the CVM Resolution 23/21 (former CVM Instruction Nº 308/99). F – Politically Exposed Persons Regarding the members of the Board of Directors, Executive Board, Fiscal Council and other Committees, we classify as politically exposed persons: Alfredo Egydio Setubal (member of the Board of Directors); Leila Cristiane Barboza Braga de Melo (member of the Executive Board), Reinaldo Guerreiro (member of the Fiscal Council) and Ricardo Baldin (member of the Audit Committee), on account of: Alfredo Egydio Setubal being characterized as a Related PEP considering the relationship with members of Liquigás Distribuidora S.A. Leila Cristiane Barboza Braga de Melo being characterized as a Related PEP considering the relationship with members of Central Bank of Brazil, Banco do Brasil and Caixa Econômica Federal. Reinaldo Guerreiro being characterized as a Related PEP considering the relationship with members of Ministry of Cities, Caixa Econômica Federal, Caixa Participações, Government of the State DF and Government of the State of RS. Ricardo Baldin be characterized as a Holder PEP considering a management position at Finame. G - ADDITIONAL INFORMATION: We inform you that the inauguration of those elected in the Annual General Stockholders’ Meeting of April 26, 2022 and meeting of the Board of Directors of April 28, 2022, was homologated by the Central Bank of Brazil on 06.21.2022 and 06.24.2022, respectively, and they took office on 07.01.2022. We inform you that the inauguration of those elected in the meeting of the Board of Directors of August 25, 2022, was homologated by the Central Bank of Brazil on 09.29.2022 and they took office

on 10.03.2022. We inform you that the inauguration of those elected in the of the Board of Directors of September 29, 2022, is pending approval of their election by the Central Bank of Brazil. Below we present the hierarchy flowchart of said Bodies: 12.5/6 – Composition and professional experience of the board of directors and fiscal council With respect to each member, see below information about item 12.5 “m”: I – Main professional experience for the past five years, indicating: Company’s name and activity sector. Position and roles inherent in the position. Whether the company is part of (i) the Issuer’s economic group or (ii) is controlled by an Issuer’s direct or indirect stockholder with an interest equal to or higher than 5% in the same class or type of the Issuer’s securities. II - All management positions they hold in other companies or third sector organizations: Company is part of (ii) is controlled by an Issuer’s direct or Company’s name Company’s activity sector indirect stockholder (i) the Issuer’s with an interest equal Other companies or economic group to or higher than 5% third sector in the same class or organizations type of the Issuer’s securities. Abril Comunicações S.A. Printing of books, magazines and other periodicals x

Alana Foundation Charitable organization x AlanaLab Communication of impact x Alpargatas S.A. Holding company of non-financial institutions x Ambev Drinks x Americel S.A. Telephony Equipment x Arthur Andersen & Co. Advocacy x Associação Brasileira das Companhias Abertas (ABRASCA - Brazilian Association of Non-profit civil association x Publicly-Held Companies) Associação Brasileira das Entidades dos Mercados Financeiro e de Capitais (ANBIMA - Non-profit civil association x Brazilian Association of Financial and Capital Market Entities) Associação Junior Achievement do Estado de Entrepreneurial Education x São Paulo Banco ABNAMRO/Real S.A. Multiple-service banking, with commercial portfolio x Banco Bandeirantes S.A. Multiple-service banking, with commercial portfolio x Banco BBA-Creditanstalt S.A. Multiple-service banking, with commercial portfolio x Banco Brascan S.A. Investment Bank x Banco Central do Brasil (BACEN - Central Bank of Brazil) Federal government agency x Banco CCF Brasil Multiple-service banking, with commercial portfolio x Banco do Brasil S.A. Multiple-service banking, with commercial portfolio x Banco HSBC Multiple-service banking, with commercial portfolio x Banco Icatu S.A. Multiple-service banking, with commercial portfolio x Banco Itaú Argentina S.A. Holding company of non-financial institutions x Banco Itaú BBA S.A. Multiple-service x Banco Itaú Chile Holding company of non-financial institutions x Banco Itaú International Holding company of non-financial institutions x Banco Itaú Paraguai Holding company of non-financial institutions x Banco Itaú Suisse Holding company of non-financial institutions x Banco Itaú Uruguai Holding company of non-financial institutions x Banco Nacional de Desenvolvimento Econômico e Social (BNDES – Brazilian Development bank x Social and Economic Development Bank) Banco PSA Finance Brasil S.A. Multiple-service banking, with commercial portfolio x Banco Santander (Brasil) S.A. Multiple-service banking, with commercial portfolio x Banco Santander S.A. Multiple-service banking, with commercial portfolio x Banco UBS Pactual Multiple-service banking, with commercial portfolio x Bank Boston Multiple-service banking, with commercial portfolio x BB Seguridade S.A. Insurance and pension plan x BM&FBOVESPA S.A. – Bolsa de Valores, Management of organized securities markets and provision Mercadorias e Futuros (currently B3 S.A. – Brasil, Bolsa, Balcão (B3 S.A. – Brazilian of services of registration, clearing and settlement, and x Exchange and OTC)) support to financing operations Bolsas y Mercados Españoles (BME) Administration of organized securities markets x Brasil Warrant Administração de Bens e Holding company of non-financial institutions x Empresas S.A (BWSA) Brazilian Chamber of Commerce in Great Non profit organization x Britain Bunge y Born Food industry x BW Gestão de Investimentos (BWGI) Management of restricted public funds x Câmara Interbancária de Pagamentos (CIP) Brazilian Payment System x Cambuhy Investimentos Ltda. Consultancy in corporate management x Carvalho Pinto, Monteiro de Barros, Frussa & Bohlsen – Advogados Accounting and tax consulting and auditing x Casa da Moeda do Brasil (CMB) Manufacture of paper money x CI&T Inc. Digital solutions x Cia. de Saneamento Básico do Estado de São Paulo (SABESP - Basic Sanitation Company of Water collection, treatment and distribution x the State of São Paulo) Cia. de Saneamento de Minas Gerais (COPASA - Minas Gerais Sanitation Water collection, treatment and distribution x Company) Cia. de Saneamento do Paraná (SANEPAR - Paraná Sanitation Company) Water collection, treatment and distribution x Citibank Brazil Financial Conglomerate x Citigroup Brasil Financial Conglomerate x Claro S.A. Telephony business x Comissão de Valores Mobiliários (CVM – Brazilian Securities and Exchange Public administration in general x Commission) Comissão Trilateral do Conselho Internacional Private Discussion Forum x da NYSE Companhia Brasileira de Distribuição S.A. Retail sales x Companhia Brasileira de Metalurgia e Mineração (CBMM - Brazilian Metallurgy and Metallurgy and technology x Mining Company) Companhia de Trens Urbanos (CBTU) Rail transport x Companhia Paranaense de Gás (COMPAGÁS - Paranaense Gas Company) Natural gas distributor x Conectas Non-governmental organization x

ConectCar Soluções de Mobilidade Eletrônica Payment Institution S.A. Conferência Monetária Internacional International Organization Conselho de Recursos do Sistema Financeiro Self-Regulatory Entity Nacional (CRSFN) Conselho Regional de Contabilidade (CRC/SP Self-Regulatory Entity - Regional Council of Accounting) CPFL Energia S.A. Energy Distribution Credicard Payment solutions Debevoise & Plimpton Advocacy Deutsche Bank Securities Investment Bank Manufacturing, sale, import, and export of wood by- Dexco S.A. products, bathroom fittings, and ceramics and plastic materials Diagnósticos da América S.A. (DASA) Clinical Diagnostics Directa Auditores Advocacy Manufacturing, sale, import, and export of wood by- Duratex S.A. products, bathroom fittings, and ceramics and plastic materials Ecorodovias Concession of Public Works and Services Manufacture and marketing of household and industrial Electrolux do Brasil S.A. appliances in general Manufacturing of intermediate products for plasticizers, Elekeiroz S.A. resins and fibers Eneva S.A. Electricity Ernst & Young Accounting and tax audit and consulting services Faculdade de Economia, Administração e Contabilidade de São Paulo (FEA-USP - School of Economics, Business Administration Higher education – undergraduate and graduate courses and Accounting of the Universidade de São Paulo) Fairplay Production and organization of corporate events Federação Brasileira de Bancos (FEBRABAN Organization of trade and business association activities – Brazilian Federation of Banks) Federação Brasileira de Bancos (FENABAN – National Federation of Banks) Employers’ union Federal Reserve Bank Federal Agency Fellow Ashoka Non-profit organization Fidelity International Ltd. Investment management Fight For Peace International Non profit organization Financeira Itaú CBD S.A. Crédito, Credit, Financing and Investment Society Financiamento e Investimento Financial Stability Board (FSB) Financial Stability Board Fortbras S.A. Automotive aftermarket Fundação Bienal de São Paulo (São Paulo Art Restoration and conservation of historical sites and Biennial Foundation) buildings Fundação das Nações Unidas (EUA) International Organization Fundação Instituto de Pesquisas Contábeis, Atuariais e Financeiras (FIPECAFI – Institute for Accounting, Actuarial and Financial Higher education – undergraduate and graduate courses Research Foundation) Activities of membership organizations related to culture Fundação Itaú para Educação e Cultura and art Fundação Itaú Unibanco Previdência Supplementary Pension Complementar Fundação Maria Cecilia Souto Vidigal Non-profit organization Fundação Nova Escola Non-profit organization Fundação OSESP (OSESP Foundation) Music teaching Fundo Garantidor de Crédito (FGC) Non-profit organization Fundo Patrimonial da FEA-USP (FEA-USP Endowment Fund) Raising and investment of funds to support FEA-USP G/xtrat Consultoria Econômica Ltda. Consultancy in corporate management Gávea Investimentos Fund management services by contract or commission GC/Capital Empreendimentos e Participações Holding company of non-financial institutions Ltda. Grant Thornton Brasil Accounting and tax consulting and auditing Grupo Boticário Cosmetics Grupo Itaú Unibanco (Itaú Unibanco Group) Financial Conglomerate Grupo Natura (Natura Group) Financial Conglomerate Gustavo Loyola Consultoria S/C Consultancy in economics Hospital de Clínicas de Porto Alegre (HCPA) Hospital care, first-aid and emergency care unit activities IBM Brasil Machinery and Services Industry Icatu Holding S.A. Holding company IFRS Foundation Production of Accounting Standards Indústrias Romi S.A. Machinery Instituto Akatu (Akatu Institute) Non-governmental organization Instituto Alana (Alana Institute) Non-governmental organization Instituto Brasileiro de Ciência Bancária (IBCB Institutes and Foundations – Brazilian Institute of Banking Science) Instituto Brasileiro de Governança Corporativa (IBGC – Brazilian Institute of Corporate Activities of associations for protection of social rights Governance)

Instituto Brasileiro de Relações com Self-Regulation Entity Investidores (IBRI) Instituto Brincante (Brincante Institute) Non-governmental organization Instituto de Arte Contemporânea Works of art Instituto de Ensino e Pesquisa (INSPER) Education institution Organization that supports entrepreneurship and Instituto Empreender Endeavor entrepreneurs Activities of associative organizations related to culture and Instituto Itaú Cultural art Instituto Lemann Pedagogical management Activities of membership organizations related to culture Instituto Unibanco and art International Integrated Reporting Committee Global authority and central coordinating body for matters (IIRC) related to Integrated Accounting Reporting Investimentos e Participações em Infraestrutura Urban mobility S.A. (INVEPAR) IRB – Brasil Resseguros S.A. Reinsurance Itaú Asset Management S.A. Fund administration Other activities of services rendered mainly to the Itau BBA International plc companies not mentioned previously Itaú Corretora de Valores S.A. Securities brokerage Itaú CorpBanca (Chile) Multiple-service banking, with commercial portfolio Other service activities provided mainly to companies not Itaú International Holding Limited previously specified Itaú Social Associations for the defense of social rights Itaú Unibanco Holding S.A. Holding company Itaú Unibanco S.A. Multiple-service banking, with commercial portfolio Itaúsa S.A. Holding company Investment in other companies in Brazil and abroad, particularly in those engaged in the manufacture and sale of Itautec S.A. banking and commercial automation equipment and provision of services IUPAR—Itaú Unibanco Participações S.A. Holding company J. P. Morgan Chase Holding company Jones Day Consulting service L. Dias Advogados Advocacy Lean Enterprise Institute (LEI) Educational Institution Luizaseg Seguros S.A. Property and casualty insurance Magazine Luiza S.A. Retail sales Mizuho International Ltd. Multiple-service banking, with commercial portfolio Mastercard Incorporated Payment Institution Metalúrgica Gerdau S.A. Steel mill Museu de Arte de São Paulo (MASP – São Non-profit private museum Paulo Art Museum) Museu de Arte Moderna de São Paulo (MAM - São Paulo Museum of Modern Art) Non-profit civil association Oi S.A. Telecommunications Ordem dos Advogados do Brasil Self-Regulatory Entity Organização Internacional das Comissões de Non-profit organization Valores (IOSCO) Pão de Açúcar – Companhia Brasileira de Retail sales Distribuição Paraná Auditores Accounting and tax audit and consulting services Parnaíba Gás Natural Extraction of oil and natural gas Wholesaling of alcohol fuel, biodiesel, gas and other oil by- Petrobrás Distribuidora S.A. products, except for lubricants, which is not carried out by a retail transportation company Petrobrás Gás S.A. (GASPETRO) Oil exploration, extraction and refining Petrobrás Transportes S.A. (TRANSPETRO) Maritime Transport Petróleo Brasileiro S.A. Oil exploration, extraction and refining Pinheiro Neto Advogados Accounting and tax audit and consulting services Porto Seguro S.A. Insurance PricewaterhouseCoopers Accounting and tax audit and consulting services Raia Drogasil Pharmaceutical Redecard S.A. Payment Institution RMB Assessoria e Consultoria Empresarial e Contábil EIRELI Business consulting Royal Institution of Great Britain Education and scientific research Sensedia Integration Solutions Serasa S.A. Information Services Sindicato dos Bancos no Estado de São Paulo Union (Union of Banks of the State of São Paulo) Spaipa S.A. Indústria Brasileira de Bebidas Beverage Manufacturing and Distribution Tecnologia Bancária (TECBAN) ATM’s Tekno S.A. Indústria e Comércio Metal Product Manufacturing Telet S.A. Telephone company Tendências Conhecimento Assessoria Econômica Ltda. Consultancy Tendências Consultoria Integrada S/S Ltda. Consultancy Tess S.A. Telephony business

Tozzini Freire Advogados Advocacy Totvs S.A. Technology Unibanco – União de Bancos Brasileiros Multiple-service banking, with commercial portfolio Universidade Estadual de Campinas Education institution (UNICAMP) Vale S.A. Mining company Verallia Glass packaging producer Ventor Investimentos Ltda. Fund management services by contract or commission W.I.L.L. – Women in Leadership in Latin International non-profit organization America XP Inc. Holding company XP Investimentos S.A. Holding company of non-financial institutions XPRIZE Non-profit organization Zup Tecnologia Technological consultancy 12.9. Existence of a marital relationship, stable union or kinship of up to second degree between: a) Issuer’s management members; b) (i) Issuer’s management members and (ii) management members of the issuer’s direct or indirect subsidiaries; c) (i) Management members of the issuer or of its direct or indirect subsidiaries and (ii) Issuer’s direct or indirect parent companies; d) (i) Issuer’s management members and (ii) management members of the Issuer’s direct or indirect parent companies; a) Issuer’s management members: • Alfredo Egydio Setubal (Member of the Board of Directors) is brother of Roberto Egydio Setubal (Co-chairman of the Board of Directors). • Joăo Moreira Salles (Member of the Board of Directors) is son of Pedro Moreira Salles (Co-chairman of the Board of Directors). • Ana Lúcia de Mattos Barretto Villela (Member of the Board of Directors) is cousin of Ricardo Villela Marino (Vice Chairman of the Board of Directors). b) (i) Issuer’s management members and (ii) management members of the issuer’s direct or indirect subsidiaries: • Not applicable. c) (i) Management members of the issuer or of its direct or indirect subsidiaries and (ii) issuer’s direct or indirect parent companies: • Pedro Moreira Salles (Co-chairman of the Board of Directors), together with siblings Fernando Roberto Moreira Salles, João Moreira Salles and Walther Moreira Salles Júnior, is in the Issuer’s controlling group; • Brothers Roberto Egydio Setubal (Co-chairman of the Board of Directors) and Alfredo Egydio Setubal (Member of the Board of Directors), together with siblings José Luiz Egydio Setubal, Olavo Egydio Setubal Júnior, Paulo Setubal Neto and Ricardo Egydio Setubal, are in the Issuer’s controlling group; • Ricardo Villela Marino (Vice Chairman of the Board of Directors), together with brother Rodolfo Villela Marino, is in the Issuer’s controlling group; • Ana Lúcia de Mattos Barreto Villela (Member of the Board of Directors), together with brother Alfredo Egydio Arruda Villela Filho, is in the Issuer’s controlling group. d) (i) Issuer’s management members and (ii) management members of the issuer’s direct or indirect parent companies: • Pedro Moreira Salles (Co-chairman of the Board of Directors), together with siblings Fernando Roberto Moreira Salles, João Moreira Salles and Walther Moreira Salles Júnior, is in the management of parent companies IUPAR – Itaú Unibanco Participações S.A. and Cia. E. Johnston de Participações;

• Joăo Moreira Salles (Member of the Board of Directors), together with father Pedro Moreira Salles (Co-chairman of the Board of Directors), is in the management of parent company IUPAR – Itaú Unibanco Participações S.A.; • Brothers Roberto Egydio Setubal (Co-chairman of the Board of Directors) and Alfredo Egydio Setubal (Member of the Board of Directors), together with brother Ricardo Egydio Setubal, are in the management of parent companies IUPAR – Itaú Unibanco Participações S.A. and Itaúsa S.A.; • Ricardo Villela Marino (Vice Chairman of the Board of Directors), together with brother Rodolfo Villela Marino, is in the management of parent company Itaúsa S.A.; • Ricardo Villela Marino (Vice Chairman of the Board of Directors), together with brother Rodolfo Villela Marino, is in the management of parent company Rudric Ith Participações Ltda., and Rodolfo Villela Marino is also a management member of the controlling company Companhia Esa; • Ana Lúcia de Mattos Barretto Villela (Member of the Board of Directors), together with brother Alfredo Egydio Arruda Villela Filho, is in the management of parent companies IUPAR – Itaú Unibanco Participações S.A. and Itaúsa S.A.; • Alfredo Egydio Setubal (Member of the Board of Directors), together with brother Ricardo Egydio Setubal, is in the management of parent company Companhia Esa. 12.10. Inform on the subordination, service provision or control relationships maintained for the last three years between the issuer’s management members: a) Issuer’s direct or indirect subsidiary, except for those in which the Issuer holds, directly or indirectly, the total capital stock; b) Issuer’s direct or indirect parent company; c) If relevant, supplier, client, debtor or creditor of the issuer, its subsidiaries or parent companies or subsidiaries of any of these people a) Issuer’s direct or indirect subsidiary, except for those in which the issuer holds, directly or indirectly, the total capital stock: Management member Ricardo Villela Marino holds a management position in subsidiaries. b) Issuer’s direct or indirect parent company: Management members Alfredo Egydio Setubal, Ana Lúcia de Mattos Barretto Villela, Pedro Moreira Salles, Ricardo Villela Marino and Roberto Egydio Setubal are part of the controlling group of Itaú Unibanco. c) If relevant, supplier, client, debtor or creditor of the issuer, its subsidiaries or parent companies or subsidiaries of any of these people: Not applicable. Other relevant information of Item 12.12 a) Regarding stockholders’ meetings held in the past three (3) years, we inform as follows: Year Type of Date/Time Quorum Stockholders’ Meeting 2022 Annual 04.26.2022 – 11:00am 92.09% of common shares and 41.35% of preferred shares 2022 Extraordinary 04.26.2022 – 11:10am 92.09% of common shares and 41.35% of preferred shares 2021 Annual and 04.27.2021 – 11:00am and 91.96% of common Extraordinary 11:10am shares and 31.67% of preferred shares 2021 Extraordinary 01.31.2021 – 11:00am More than 90% of common shares

2020Annual04.28.2020 – 11:00amMore than 90% of common shares and 31.75% of preferred shares 2019Annual04.24.2019 – 11:00amMore than 90% of common shares and 29.08% of preferred shares b) Audit Committee: The Audit Committee has independence to set and engage training activities. In 2016, the Audit Committee started defining, twice a year, the need for training identified as significant for its performance. Once it identifies a department in need of training, it engages training services to meet a specific need for this department or its members. Another training component of the Audit Committee for topics under its responsibility it understands as significant is benchmarking with other organizations, including abroad, or with the best practices identified by consultants. In 2019, the Audit Committee promoted specific anti-money laundering (AML) training with the attendance of members of the Committee and guests representing the Internal Audit (Brazil and Latam) and Operating Risk Offices. Talks were given by consultants in Brazil and abroad on (i) Anti money laundering and combating terrorism financing (AML/CFT) and Anti-bribery and corruption (AB&C); and (ii) The Evolving Landscape of AML/CFT Management. In 2019, 2020 and 2021, some of its members, individually, also participated in training sessions on accounting, corporate governance, ethics, financial and external audit related topics. c) Relationship among the Audit Committee, the Board of Officers, and the Co- chairpersons of the Board of Directors Based on the responsibilities set out in its Regulations and the assessment of main risks of the Itaú Unibanco Conglomerate, the Audit Committee annually defines its meeting schedule, including with the Board of Officers. This annual planning is continuously revised by the Audit Committee, which may change its meeting planning at any time. Throughout 2019, 2020 and 2021, the Audit Committee held meetings at least on a monthly basis with the executives in charge of the Internal Audit, Compliance and Operating Risk and Internal Control departments, to monitor the outcomes of the work carried out by these departments, as well as bimonthly meetings to monitor the operation of Itaú CorpBanca in Chile and its subsidiaries. Also during these years, the Audit Committee held meetings with the following departments: Finance, Corporate Security, Retail Banking, Wholesale Banking, Technology and Operations, Credit Risk, Market and Liquidity Risk, Legal, External Ombudsman’s Office, Internal Ombudsman’s Office, and with those responsible for a number of business of Itaú Unibanco Conglomerate, including abroad, covering Itaú Unibanco’s units in Latin America and in the Northern hemisphere (the U.S. and the Caribbean, Europe, Asia and Middle East). At least quarterly, since 2021 the Audit Committee has held a private meeting with the CEO of Itaú Unibanco Holding S.A., and has continued to hold, likewise for a number of years, a joint meeting with the with the Co-chairpersons of the Board of Directors and with the CEO of Itaú Unibanco Holding S.A., in which the Audit Committee submits its findings and recommendations and monitors the progress of previously submitted recommendations. Relationship among the Audit Committee, the Board of Directors and the Supervisory Council The Audit Committee reports to the Board of Directors of Itaú Unibanco Holding S.A. On a quarterly basis, the Chairperson of the Audit Committee submits to the Board of Directors a summary of the most significant topics discussed at the meetings held in the period. On a semi-annual basis,

the Audit Committee submits its recommendations on the financial statements, and it annually submits the outcome of the evaluation by the external auditor, internal auditor and operating risk department, which is also responsible for the internal controls. The Audit Committee holds a joint meeting with the members of the Supervisory Council of Itaú Unibanco Holding SA at least once a year, in which it submits its findings on Itaú Unibanco’s consolidated financial statements for the year ended in December of each year or other topics of interest of the Supervisory Council. Relationship between the Board of Directors and the Supervisory Council The Supervisory Council attends the Board of Directors’ meeting in which the Issuer’s annual financial statements are examined (therefore, once a year). Relationship between the Supervisory Council and the Board of Officers The Supervisory Council meets the Board of Officers of Itaú Unibanco Holding S.A. when the Issuer’s financial statements are presented (therefore, four times a year). Relationship between the Board of Directors and the Investor Relations Officer The main relationship channel between the Board of Directors and the Investor Relations Officer is the Disclosure and Trading Committee. This committee meets every quarter on a mandatory basis, in addition to approving Material Facts and Announcements to the Market, among other materials, on a timely basis. The composition of the Disclosure and Trading Committee strengthens the relationship with the Board of Directors, since it is composed of members of the Board of Directors, the Executive Committee, and the Board of Officers. Noteworthy is that the topics included in the agenda of the Disclosure and Trading Committee’s meetings may be directly related to the Board of Directors or the Statutory Committees reporting to the Board of Directors, such as: Management Report, Form 20-F, Reference Form, and Integrated Annual Report; Changing and creating new policies; Opinions on the performance of Itaú Unibanco’s securities and the best practices from market participants, including investors, ESG* and credit rating agencies, corporate governance, analysts, and trade associations; Share bonus and share splits; Analyzing the trades made by the parties adhering to the Securities Trading Policy. The Itaú Unibanco’s Investor Relations Officer also prepares materials to the Board of Directors, comparing the financial performance of Itaú Unibanco with that of its main competitors, in addition to calculating the market share of the key products of the Bank and its subsidiaries. *Stands for Environmental, Social and Corporate Governance

d) In 2021, we developed the following training activities: Ethics and Up to Compliance officers Biennial 93% Up to Anti-corruption Biennial 95% officers Adherence to Up to Board of Annual 96% Code of Ethics Directors Anti-Money Laundering and Up to officers Biennial 96% Counter Terrorist Financing E-learning on the Up to executive theme of Biennial 91.55% sustainability officers E-learning focused on the guidelines of the Supplier Up to executive Relationship Biennial 93.12% Code, which is officers part of the Integrity and Ethics Program. Online event “Meeting Itaú + Suppliers” covering the topics: ethics and corruption prevention, prevention of Critical suppliers Annual 51.11% money laundering and fighting the financing of terrorism, relationship conflicts and ombudsman. Note: biannually a lecture on the Prevention of Illicit Acts is given to the Board of Directors, as well as reinforcement of the corruption prevention training to specific areas. e) In 2021, the Internal Ombudsman’s Office received 2,211 calls related to inter-personal conflicts and conflicts of interest in the workplace involving the organization’s employees. In order to guide and discipline any employees who show conduct contrary to the principles of the Company’s Code of Ethics and standards, and to minimize any related risks, the Company has implemented guiding/disciplinary measures to those involved in calls investigated and deemed as legitimate. Additionally, a number of monitoring actions and action and development plans were recommended to any reported employees. f) Supporting documentation for the meetings of the Board of Directors:

The members of the Board of Directors receive, at least five (5) days before the meeting, whenever possible, supporting documents for the topics that will be discussed, so that each Director may be properly aware of these topics and be prepared for a productive cooperation in these debates. g) Information related to the evaluation process of the Board of Directors, Committees and Executive Board is described in item 12.1.c

ITEM 15. CONTROL AND ECONOMIC GROUP BASE-DATE 10.06.2022 Ordinary Preferrend Itaú Unibanco Holding S.A. % % Total % Shares Shares IUPAR - Itaú Unibanco Participações S.A. 2.564.084.404 51,713 - - 2.564.084.404 26,153 Brazilian CNPJ 04.676.564/0001-08 Itaúsa S.A. 1.943.906.577 39,205 169.323 0,003 1.944.075.900 19,829 Brazilian CNPJ 61.532.644/0001-15 BlackRock, INC - - 349.925.097 7,221 349.925.097 3,569 American GQG Partners LLC - - 253.506.105 5,231 253.506.105 2,586 American Treasury Shares - - 3.268.688 0,067 3.268.688 0,033 Others 450.299.378 9,082 4.238.975.776 87,477 4.689.275.154 47,830 Total 4.958.290.359 100,000 4.845.844.989 100,000 9.804.135.348 100,000 BASE-DATE 12.31.2021 Ordinary Preferrend IUPAR - Itaú Unibanco Part. S.A. % % Total % Shares Shares Itaúsa S.A. 355,227,092 50.000 350,942,273 100.000 706,169,365 66.532 Brazilian CNPJ 61.532.644/0001-15 Cia. E. Johnston de Participações 355,227,092 50.000 - - 355,227,092 33.468 Brazilian CNPJ 04.679.283/0001-09 Total 710,454,184 100.000 350,942,273 100.000 1,061,396,457 100.000 BASE-DATE 12.31.2021 Ordinary Preferrend Cia. E. Johnston de Part. % % Total % Shares Shares Fernando Roberto Moreira Salles 2,030,000 31.002 4,060,000 31.002 6,090,000 31.002 Brazilian CPF(*) 002.938.068-53 João Moreira Salles 1,244,000 18.998 2,488,000 18.998 3,732,000 18.998 Brazilian CPF(*) 667.197.397-00 Pedro Moreira Salles 2,030,000 31.002 4,060,000 31.002 6,090,000 31.002 Brazilian CPF(*) 551.222.567-72 Walther Moreira Salles Júnior 1,244,000 18.998 2,488,000 18.998 3,732,000 18.998 Brazilian CPF(*) 406.935.467-00 Total 6,548,000 100.000 13,096,000 100.000 19,644,000 100.000

BASE-DATE 10.04.2022 Ordinary Preferrend BA Itaúsa S.A. % % Total % Shares Shares ESA Company 19.080.102 0,629 —   —   19.080.102 0,216 Itaú Brazilian ESA CNPJ 52.117.397/0001-08 Braz Fundação Itaú Para a Educação e Cultura 354.562.905 11,685 43.546.977 0,751 398.109.882 4,508 CNP Brazilian —   Fun CNPJ 59.573.030/0001-30 —   Braz Fundação Antonio e Helena Zerrenner CNP Instituição Nacional de Beneficência 466.488.268 15,374 116.261.731 2,006 582.749.999 6,599 Fun Brazilian —   Insti CNPJ 60.480.480/0001-67 —   Braz O. E. Setubal S.A. 6 0,001 8 0,001 14 0,001 CNP Brazilian —   O. E CNPJ 61.074.456/0001-90 —   Braz Rudric ITH Participações Ltda. 2.530.433 0,083 1.997.577 0,034 4.528.010 0,051 CNP Brazilian —   Rud CNPJ 67.569.061/0001-45 —   Braz Alfredo Egydio Arruda Villela Filho 387.520.211 12,771 226.975.746 3,915 614.495.957 6,958 CNP Brazilian —   Alfr CPF(*) 066.530.838-88 —   Braz Ana Lúcia de Mattos Barreto Villela 387.520.184 12,771 211.756.213 3,653 599.276.397 6,786 CPF Brazilian —   Ana CPF(*) 066.530.828-06 —   Braz Ricardo Villela Marino 193.979.937 6,393 149.813.689 2,584 343.793.626 3,893 CPF Brazilian —   Rica CPF(*) 252.398.288-90 —   Braz CPF Rodolfo Villela Marino 194.037.513 6,395 149.907.666 2,586 343.945.179 3,895 Brazilian —   CPF(*) 271.943.018-81 —   Paulo Setubal Neto 33.112 0,001 26.626.937 0,459 26.660.049 0,302 Brazilian —   CPF(*) 638.097.888-72 —   Carolina Marinho Lutz Setubal 41.228.062 1,359 5.320.159 0,092 46.548.221 0,527 Brazilian —   CPF(*) 077.540.228-18 —   Julia Guidon Setubal Winandy 41.228.062 1,359 5.320.159 0,092 46.548.221 0,527 Brazilian —   CPF(*) 336.694.358-08 —   Paulo Egydio Setubal 41.228.062 1,359 5.320.159 0,092 46.548.221 0,527 Brazilian —   CPF(*) 336.694.318-10 —   Fernando Setubal Souza e Silva 22.421.320 0,739 9.286.423 0,160 31.707.743 0,359 Brazilian —   CPF(*) 311.798.878-59 —   Guilherme Setubal Souza e Silva 22.421.433 0,739 8.855.932 0,153 31.277.365 0,354 Brazilian —   CPF(*) 269.253.728-92 —   Tide Setubal Souza e Silva Nogueira 22.421.780 0,739 9.887.507 0,171 32.309.287 0,366 Brazilian —   CPF(*) 296.682.978-81 —   Olavo Egydio Setubal Júnior 10.992.607 0,362 44.209.013 0,763 55.201.620 0,625 Brazilian —   CPF(*) 006.447.048-29 —  

Bruno Rizzo Setubal 32.839.996 1,082 19.946 0,001 32.859.942 0,372 Brazilian CPF(*) 299.133.368-56 Camila Setubal Lenz Cesar 32.839.997 1,082 22.249 0,001 32.862.246 0,372 Brazilian CPF(*) 350.572.098-41 Luiza Rizzo Setubal Kairalla 32.840.000 1,082 29.334 0,001 32.869.334 0,372 Brazilian —   CPF(*) 323.461.948-40 —   Roberto Egydio Setubal 56.581.974 1,865 21.209.080 0,366 77.791.054 0,881 Brazilian —   CPF(*) 007.738.228-52 —   Mariana Lucas Setubal 26.252.185 0,865 9.840.829 0,170 36.093.014 0,409 Brazilian —   CPF(*) 227.809.998-10 —   Paula Lucas Setubal 26.252.185 0,865 9.840.829 0,170 36.093.014 0,409 Brazilian —   CPF(*) 295.243.528-69 —   José Luiz Egydio Setubal 93.676.269 3,087 41.288.277 0,712 134.964.546 1,528 Brazilian —   CPF(*) 011.785.508-18 —   Beatriz de Mattos Setubal 4.953.480 0,163 302.863 0,005 5.256.343 0,060 Brazilian —   CPF(*) 316.394.318-70 —   Gabriel de Mattos Setubal 4.953.480 0,163 302.863 0,005 5.256.343 0,060 Brazilian —   CPF(*) 348.338.808-73 —  

Olavo Egydio Mutarelli Setubal 4.953.480 0,163 302.863 0,005 5.256.343 0,060 Brazilian —   CPF(*) 394.635.348-73 —   Alfredo Egydio Setubal 108.581.745 3,578 43.145.428 0,744 151.727.173 1,718 Brazilian —   CPF(*) 014.414.218-07 —   Alfredo Egydio Nugent Setubal 2.185 0,001 220 0,001 2.405 0,001 Brazilian CPF(*) 407.919.708-09 Marina Nugent Setubal 2.185 0,001 220 0,001 2.405 0,001 Brazilian —   CPF(*) 384.422.518-80 —   Ricardo Egydio Setubal 108.532.016 3,577 43.542.330 0,751 152.074.346 1,722 Brazilian —   CPF(*) 033.033.518-99 —   Marcelo Ribeiro do Valle Setubal 2.218 0,001 305.518 0,005 307.736 0,003 Brazilian —   CPF(*) 230.936.378-21 —   Rodrigo Ribeiro do Valle Setubal 2.218 0,001 287.748 0,005 289.966 0,003 Brazilian —   CPF(*) 230.936.298-02 —   Patricia Ribeiro do Valle Setubal 2.218 0,001 305.518 0,005 307.736 0,003 Brazilian — CPF(*) 230.936.328-62 —   BlackRock, Inc —   —   290.508.650 5,011 290.508.650 3,290 American —   Treasury 3.492.300 0,115 8.400.000 0,145 11.892.300 0,135 Others 289.875.531 9,549 4.312.285.357 74,384 4.602.160.888 52,107 Total 3.034.329.659 100,000 5.797.026.018 100,000 8.831.355.677 100,000

BASE-DATE 12.31.2021 Ordinary ESA Company % Total % Shares O. E. Setubal S.A. 6 0.001 6 0.001 Brazilian CNPJ 61.074.456/0001-90 Rudric ITH Participações Ltda. 2,530,433 0.133 2,530,433 0.133 Brazilian CNPJ 67.569.061/0001-45 Alfredo Egydio Arruda Villela Filho 387,520,211 20.385 387,520,211 20.385 Brazilian CPF(*) 066.530.838-88 Ana Lúcia de Mattos Barreto Villela 387,520,184 20.382 387,520,184 20.382 Brazilian CPF(*) 066.530.828-06 Ricardo Villela Marino 193,979,937 10.205 193,979,937 10.205 Brazilian CPF(*) 252.398.288-90 Rodolfo Villela Marino 194,037,513 10.208 194,037,513 10.208 Brazilian CPF(*) 271.943.018-81 Paulo Setubal Neto 33,112 0.002 33,112 0.002 Brazilian CPF(*) 638.097.888-72 Carolina Marinho Lutz Setubal 41,228,062 2.169 41,228,062 2.169 Brazilian CPF(*) 077.540.228-18 Julia Guidon Setubal Winandy 41,228,062 2.169 41,228,062 2.169 Brazilian CPF(*) 336.694.358-08 Paulo Egydio Setubal 41,228,062 2.169 41,228,062 2.169 Brazilian CPF(*) 336.694.318-10 Fernando Setubal Souza e Silva 22,421,320 1.180 22,421,320 1.180 Brazilian CPF(*) 311.798.878-59

Guilherme Setubal Souza e Silva 22,421,433 1.180 22,421,433 1.180 Brazilian CPF(*) 269.253.728-92 Tide Setubal Souza e Silva Nogueira 22,421,780 1.180 22,421,780 1.180 Brazilian CPF(*) 296.682.978-81 Olavo Egydio Setubal Júnior 10,992,607 0.578 10,992,607 0.578 Brazilian CPF(*) 006.447.048-29 Bruno Rizzo Setubal 32,839,996 1.728 32,839,996 1.728 Brazilian CPF(*) 299.133.368-56 Camila Setubal Lenz Cesar 32,839,997 1.728 32,839,997 1.728 Brazilian CPF(*) 350.572.098-41 Luiza Rizzo Setubal Kairalla 32,840,000 1.728 32,840,000 1.728 Brazilian CPF(*) 323.461.948-40 Roberto Egydio Setubal 56,581,974 2.977 56,581,974 2.977 Brazilian CPF(*) 007.738.228-52 Mariana Lucas Setubal 26,252,185 1.381 26,252,185 1.381 Brazilian CPF(*) 227.809.998-10 Paula Lucas Setubal 26,252,185 1.381 26,252,185 1.381 Brazilian CPF(*) 295.243.528-69 José Luiz Egydio Setubal 93,676,269 4.928 93,676,269 4.928 Brazilian CPF(*) 011.785.508-18 Beatriz de Mattos Setubal 4,953,480 0.261 4,953,480 0.261 Brazilian CPF(*) 316.394.318-70 Gabriel de Mattos Setubal 4,953,480 0.261 4,953,480 0.261 Brazilian CPF(*) 348.338.808-73 Olavo Egydio Mutarelli Setubal 4,953,480 0.261 4,953,480 0.261 Brazilian CPF(*) 394.635.348-73 Alfredo Egydio Setubal 108,581,745 5.712 108,581,745 5.712 Brazilian CPF(*) 014.414.218-07

Alfredo Egydio Nugent Setubal 2,185 0.001 2,185 0.001 Brazilian CPF(*) 407.919.708-09 Marina Nugent Setubal 2,185 0.001 2,185 0.001 Brazilian CPF(*) 384.422.518-80 Ricardo Egydio Setubal 108,532,016 5.710 108,532,016 5.710 Brazilian CPF(*) 033.033.518-99 Marcelo Ribeiro do Valle Setubal 2,218 0.001 2,218 0.001 Brazilian CPF(*) 230.936.378-21 Patricia Ribeiro do Valle Setubal 2,218 0.001 2,218 0.001 Brazilian CPF(*) 230.936.328-62 Rodrigo Ribeiro do Valle Setubal 2,218 0.001 2,218 0.001 Brazilian CPF(*) 230.936.298-02 Total 1,900,830,553 100.000 1,900,830,553 100.000 BASE-DATE 12.31.2021 Ordinary O. E. Setubal S.A. % Total % Shares Paulo Setubal Neto 100,000 14.286 100,000 14.286 Brazilian CPF(*) 638.097.888-72 Maria Alice Setubal 100,000 14.286 100,000 14.286 Brazilian CPF(*) 570.405.408-00 Olavo Egydio Setubal Júnior 100,000 14.285 100,000 14.285 Brazilian CPF(*) 006.447.048-29 Roberto Egydio Setubal 100,000 14.286 100,000 14.286 Brazilian CPF(*) 007.738.228-52 José Luiz Egydio Setubal 100,000 14.285 100,000 14.285 Brazilian CPF(*) 011.785.508-18 Alfredo Egydio Setubal 100,000 14.286 100,000 14.286 Brazilian CPF(*) 014.414.218-07 Ricardo Egydio Setubal 100,000 14.286 100,000 14.286 Brazilian CPF(*) 033.033.518-99 Total 700,000 100.000 700,000 100.000

BASE-DATE 12.31.2021 Ordinary Rudric ITH Participações Ltda. % Total % Shares Ricardo Villela Marino 37,507,724 50.000 37,507,724 50.000 Brazilian CPF(*) 252.398.288-90 Rodolfo Villela Marino 37,507,724 50.000 37,507,724 50.000 Brazilian CPF(*) 271.943.018-81 Total 75,015,448 100.000 75,015,448 100.000 (*) Individual Taxpayer’s Registry (CPF)

15.3. - Distribution of Capital Date of last general stockholders’ meeting/ Date of last update 04/26/2022 Number of stockholders - individuals (units) 458,530 Number of stockholders - companies (units) 12,734 Number of institutional investors (units) 1,548 Outstanding shares Outstanding shares correspond to the Issuer’s total shares, except for those held by the parent company, the people related to the latter, the Issuer’s management members, and treasury shares. Number of common shares (units) 384,272,066 7.750% Number of preferred shares (units) 4,801,489,940 99.085% Total 5,185,762,006 52.894%

15.4. Please insert a flowchart of the issuer’s stockholders and the economic group in which the issueris included, identifying: a) all direct and indirect controlling stockholders, and, should the issuer wish, stockholders owning aninterest equal to or higher than 5% of a class or type of shares (1) Date: 03.31.2022. 0.01% (2) Date: 10.06.2022. (3) Date: 04.26.2022. Family Egydio de Souza Family Moreira Salles Free Float (1) (*) Aranha (1) 63.27% common shares 36.61% common shares 18.08% preferred shares 81.70% preferred shares 100.00% Total 33.61% Total 66.21% Total Cia. E . Johnston Itaúsa S.A. de Participações 50.00% common shares 100.00% preferred shares 66.53% Total IUPAR - Itaú Unibanco Free Float (*) 50.00 % common shares Participações 39.21% common shares 33.47% Total 19.83% Total 51.71% common shares Itaú Unibanco 7.75% common shares 26.15% Total Holding S.A. 99.08% preferred shares 52.89% Total (2) (3) 97.61% common shares 99.99% common shares 100.00% common shares 100.00% Total 79.07% preferred shares 100.00% preferred shares 100.00% preferred shares 26.30% Total 97.61% Total 99.99% Total 100.00% Total Itaú Consultoria Banco Itaú Itaú CorpBanca S.A. IGA Participações S.A. Banco Itaú BBA S.A. de Valores Mobiliários Uruguay S.A. e Participações S.A. 11.12% common shares 12.65% common shares 10.75% preferred shares 12.65% preferred shares 11.12%Total 12.65%Total Itaú Rent Itaú Corretora de Administração e Seguros S. A. Participações Ltda. 100.00% common shares 99.99% common shares 99.99% common shares 100.00% Total 100.00% preferred shares 100.00% preferred shares 100.00% preferred shares 100.00% Total 100.00% Total 99.99% Total 99.99% Total 0.01% 0.01% common Itaú Corretora common OCA S.A. Bicsa Holdings, Ltd. Itaú Unibanco S.A. shares shares Banco Itaucard S.A. 0.00% de Valores S.A. 0.00% preferred preferred shares shares (0.01% Total 0.01% Total 1(1) Date: 03.31.2022. ((2) Date: 10.06.2022. The percentages do not include treasury shares. (*) In addition to treasury shares, percentages do not include the interest held by controlling stockholders. (3) Date: 04.26.2022. Direct subsidiaries

a) Direct and indirect controlling stockholders Direct controlling stockholders Itaúsa S.A. IUPAR—Itaú Unibanco Participações S.A. lndirect controlling stockholders Alfredo Egydio Arruda Villela Filho Alfredo Egydio Nugent Setubal Alfredo Egydio Setubal Ana Lúcia de Mattos Barretto Villela Beatriz de Mattos Setubal Bruno Rizzo Setubal Camila Setubal Lenz Cesar Carolina Marinho Lutz Setubal Cia. E.Jonhston de Participações Companhia ESA Fernando Roberto Moreira Salles Fernando Setubal Souza e Silva Gabriel de Mattos Setubal Guilherme Setubal Souza e Silva João Moreira Salles José Luiz Egydio Setubal Julia Guidon Setubal Winandy Luiza Rizzo Setubal Kairalla Marcelo Ribeiro do Valle Setubal Mariana Lucas Setubal Marina Nugent Setubal O.E. Setubal S.A. Olavo Egydio Setubal Júnior Olavo Egydio Mutarelli Setubal Patrícia Ribeiro do Valle Setubal Paula Lucas Setubal Paulo Egydio Setubal Paulo Setubal Neto Pedro Moreira Salles Ricardo Egydio Setubal Ricardo Villela Marino Roberto Egydio Setubal Rodolfo Villela Marino Rodrigo Ribeiro do Valle Setubal Rudric ITH Participações Ltda. Tide Setubal Souza e Silva Nogueira Walther Moreira Salles Júnior

b) Subsidiary and affiliated companies c) Issuer’s ownership interest in the group companies d) Group companies’ ownership interest in the issuer e) Companies under common control ln Brazil Itaú Unibanco S.A. Banco Itaú BBA S.A. Banco Itaucard S.A. Itaú Consultoria de Valores Mobiliários e Participações S.A. Itaú Corretora de Valores S. A. Itaú Corretora de Seguros S. A. Itaú Rent Administração e Participações Ltda. IGA Participações S.A. Itaú Seguros S.A. ITB Holding Brasil Participações Ltda. Abroad Itaú Corpbanca Banco Itaú Uruguay S.A. Bicsa Holdings, Ltd. OCA S.A. lnterest in voting capital (%) 100.00 99.99 99.99 100.00 99.99 11.12 12.65 97.61 0.00 0.00 26.30 100.00 100.00 100.00 lnterest in capital (%) 100.00 99.99 99.99 100.00 99.99 10.75 12.65 79.07 0.00 0.00 26.30 100.00 100.00 100.00 Subsidiary or affiliated company Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary Subsidiary

15.8. Other significant information Additional information on items 15.1/15.2 a) Regarding the stockholding position of stockholder BlackRock, Inc. (“BlackRock”), the Company informs that on March 3, 2011 it received the information, as provided for in Article 12, of CVM Resolution No. 44/2021, as amended by CVM Instruction No. 568/2015, that, as investment manager of some of its clients, BlackRock acquired 159,335,737 preferred shares issued by the Company. Considering the several corporate events in the Company since the interest acquisition, we present below the changes in the BlackRock’s stockholding position, which represents 7.221% preferred shares and 3.569% of BlackRock’s total capital. Statement of Changes in Blackrock’s Stockholding Position (*) Ownership interest at base date 08.19.2010 provided by the Stockholder on March 30, 2011 b) Regarding the shareholding position of the shareholder Dodge & Cox, the company informs that on October 6, 2010, it received information, in accordance with Article 12, CVM Resolution 44/2021, as amended by ICVM 568/2015, that Dodge & Cox, as the investment manager of some of its clients, now holds 241,984,749 preferred shares issued by the company. Considering the corporate event informed, below is the statement of Dodge & Cox’s shareholding evolution, which represents 4.994% of the preferred shares and 2.468% of the total capital. DATE EVENT OPENING EVENT BALANCE 03/30/2011 Opening balance at 03.30.2011, 159,335,737 as provided by Blackrock (*) CLOSING BALANCE 159,335,737 11/01/2011 Stock split/reverse split 159,335,737 159,335,700 159,335,700 according to notice of 09.01.2011 07.27.2018) 04/19/2013 10% Bonus Share (ASM of 159,335,700 15,933,570 175,269,270 04.19.2013) 06/11/2014 10% Bonus Share (ASM of 175,269,270 17,526,927 192,796,197 04.23.2014) 07/31/2015 10% Bonus Share (ASM of 192,796,197 19,279,620 212,075,817 04.29.2015) 10/21/2016 10% Bonus Share (ASM of 212,075,817 21,207,581 233,283,398 09.14.2016) 11/26/2018 Stock split according to notice 233,283,398 116,641,699 349,925,097 of 11.01.2018 (ASM of 07.27.2018)

Statement of Changes in Dodge & Cox’s Stockholding Position 10/06/2022 Opening balance at 10/06/2022, as - 783,500 241,984,749 provided by Dodge & Cox (**) (**) Ownership interest at base date 10.06.2022 provided by the Stockholder on the same date. c) Regarding the stockholding position of stockholder GQG Partners LLC, the Company informs that on January 18, 2022, it received the information, as provided for in Article 12, of CVM Resolution No. 44/2021, that GQG Partners LLC acquired 253,506,105 preferred shares issued by the Company. Considering the corporate event mentioned above, we present below the changes in the GQG Partners LLC’s stockholding position, which represents 5.231% preferred shares and 2.586% of GQG Partners LLC’s total capital. Statement of Changes in GQG Partners LLC’s Stockholding Position (***) Ownership interest at base date 01.18.2022 provided by the Stockholder on January 18, 2022 DATE 01/18/2022 EVENT Opening balance at 01.18.2022, as provided by GQG Partners LLC (***) OPENING BALANCE 253,506,105 EVENT —   CLOSING BALANCE 253,506,105 d) As of February 24, 2022, capital stock of Cia. E. Johnston de Participações “EJ”, currently distributed among brothers Fernando Roberto Moreira Salles, Walther Moreira Salles Jr., Pedro Moreira Salles and João Moreira Salles, will be shared by Fernando Roberto Moreira Salles, who will hold 50% of EJ’s capital, and Pedro Moreira Salles and son, João Moreira Salles, who will hold 44% and 6% of EJ’s capital, respectively. Pedro Moreira Salles and João Moreira Salles are also members of Itaú Unibanco’s Board of Directors. Brothers Walther Moreira Salles Jr. and João Moreira Salles will therefore no longer be stockholders of EJ, thus transferring their respective interests to the remaining stockholders, Fernando and Pedro, and to the new stockholder, João, through stock trading transactions, the completion of which is subject to the approval from the Central Bank of Brazil. Equity interest held by EJ in IUPAR’s capital stock, the controlling stockholder of Itaú Unibanco Holding, will remain unchanged. The control of IUPAR will continue to be exercised in accordance with the stockholders’ agreement in force and consequently the transactions described above will not change Itaú Unibanco Holding’s management or governance. Additional information on item 15.3 The number of individual and corporate stockholders and institutional investors stated in item 15.3 hereof refers to the base date of April 26, 2022.

The number of outstanding shares stated in item 15.3 hereof refers to the base date of October 06, 2022.

19.2 - In relation to securities held in treasury, in table format, segregated by kind, class, and type, indicate: On 01/18/2022, the item 15.1/2 - Shareholding Position was updated and, as a result, we made available a line with the movement of securities held in treasury on the base date 01/18/2022. The Annual General Stockholders’ Meeting and the Extraordinary General Stockholder’s Meeting was held on 04/26/2022, we made available a line informing the event. On 10/06/2022 occurred a Announcements to the Market of relevant participation, we made available a line informing the event.

19.3. Other significant information Additional information on items 19.2 19.2—In relation to securities held in treasury, in table format, segregated by kind, class, and type, indicate: On 01/18/2022, the item 15.1/2—Shareholding Position was updated and, as a result, we made available a line with the movement of securities held in treasury on the base date 01/18/2022. The Annual General Stockholders’ Meeting and the Extraordinary General Stockholder’s Meeting was held on 04/26/2022, we made available a line informing the event. On 10/06/2022 occurred a Announcements to the Market of relevant participation, we made available a line informing the event. a. opening number; b. acquired number; c. weighted average purchase price; d. number sold; e. weighted average sale price; f. number cancelled; g. closing number; h. percentage in relation to outstanding securities of the same class and type. Changes Opening balance Acquisition Disposal Cancellation Closing balance Changes Opening balance Acquisition (*) Disposal Cancellation Stock split Closing balance (*) Repurchase amounts include settlement, brokerage and trading fees Common Shares Numbers (Units) —   —   —   —   —   Preferred shares Numbers (Units) 3,639,091 —   (370,403) —   —   3,268,688 10/06/2022 Weighted average price (R$) Weighted average price (R$) R$ 21.76 % in relation to outstanding securities of the same class and 0.0% 0.0% % in relation to outstanding securities of the same class and 0.1% 0.1% Changes Opening balance Acquisition Disposal Cancellation Closing balance Changes Opening balance Acquisition (*) Disposal Cancellation Stock split Closing balance (*) Repurchase amounts include settlement, brokerage and trading fees Common Shares Numbers (Units) —   —   —   —   —   Preferred shares Numbers (Units) 24,244,725 —   (20,605,634) —   —   3,639,091 04/26/2022 Weighted average price (R$) Weighted average price (R$) R$ 21.76 % in relation to outstanding securities of the same class and type 0.0% 0.0% % in relation to outstanding securities of the same class and type 0.5% 0.1%

01/18/2022 Common Shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance —   0.0% Acquisition —   Disposal —   Cancellation —   Closing balance —   0.0% Preferred shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance 24,244,725 0.5% Acquisition (*) —   Disposal —   Cancellation —   Stock split —   Closing balance 24,244,725 0.5% (*) Repurchase amounts include settlement, brokerage and trading fees

12/31/2021 Common Shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance —   0.0% Acquisition —   Disposal —   Cancellation —   Closing balance —   0.0% Preferred shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance 41,678,452 0.9% Acquisition (*) —   Disposal (17,433,727) R$ 21.76 Cancellation —   Stock split —   Closing balance 24,244,725 0.5% (*) Repurchase amounts include settlement, brokerage and trading fees 12/31/2020 Common Shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance —   0.0% Acquisition —   Disposal —   Cancellation —   Closing balance —   0.0% Preferred shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance 58,533,585 1,2% Acquisition (*) —   Disposal (16,855,133) R$ 21.76 Cancellation —   Stock split —   Closing balance 41,678,452 0.9% (*) Repurchase amounts include settlement, brokerage and trading fees 12/31/2019 Common Shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance —   0.0% Acquisition —   Disposal —   Cancellation —   Closing balance —   0.0% Preferred shares Weighted average price % in relation to outstanding Changes Numbers (Units) securities of the same class and (R$) type Opening balance 83,614,426 1.8% Acquisition (*) —   Disposal (25,080,841) R$ 21.76 Cancellation —   Stock split —   Closing balance 58,533,585 1,2% (*) Repurchase amounts include settlement, brokerage and trading fees