Exhibit 99.1

DouYu International Holdings Limited Reports Unaudited Financial Results For Fourth Quarter and Full Year 2020

WUHAN, China, March 23, 2021 /PRNewswire/ — DouYu International Holdings Limited (“DouYu” or the “Company”) (Nasdaq: DOYU), a leading game-centric live streaming platform in China and a pioneer in the eSports value chain, today announced its unaudited financial results for the fourth quarter and the full year ended December 31, 2020.

Fourth Quarter 2020 Financial and Operational Highlights

Full Year 2020 Financial Highlights

Mr. Shaojie Chen, Chief Executive Officer of DouYu, commented, “As a leading game-centric live streaming platform in China, we continued to deliver strong operational results as our average mobile MAUs increased by 6.9% year over year to 58.2 million in the fourth quarter. Additionally, by the end of 2020, the improvements we had made to our video and community segments enabled us to establish a fully integrated content ecosystem on top of our leading live streaming business. This improvement has laid a solid foundation for us to further develop our diversified and integrated game-centric content platform.”

Mr. Hao Cao, Vice President of DouYu, commented, “We maintained our strong financial performance in the full year of 2020 as our total net revenues increased by 32% year over year to RMB 9.6 billion . Meanwhile, adjusted net income was RMB 541.6 million and adjusted net margin was 5.6%, representing an increase of approximately 80 basis points from last year. Looking ahead, we will continue to explore improvements for our platform’s monetization capabilities, enhance our monetization efficiency, and further utilize our operating leverage to bring more long-term value to our shareholders.”

Fourth Quarter 2020 Financial Results

Total net revenues in the fourth quarter of 2020 increased by 10.0% to RMB2,269.2 million (US$347.8 million) from RMB2,062.9 million in the same period of 2019, primarily driven by the increase in live streaming revenues.

Live streaming revenues in the fourth quarter of 2020 increased by 9.4% to RMB2,070.7 million (US$317.3 million) from RMB1,892.5 million in the same period of 2019. This increase was primarily attributable to an increase in paying users and ARPPU driven by improved user paying experience, which resulted from the Company’s ongoing product refinement efforts, as well as the Company’s cultivation of user paying habits through expansion of paying scenarios.

Advertising and other revenues in the fourth quarter of 2020 increased by 16.5% to RMB198.5 million (US$30.4 million) from RMB170.4 million in the same period of 2019, primarily attributable to the Company’s enhanced brand recognition and the corresponding increase in advertising demand for the Company’s competitive advertising and promotion solutions.

Cost of revenues in the fourth quarter of 2020 increased by 23.7% to RMB2,087.0 million (US$319.9 million) from RMB1,687.7 million in the same period of 2019, primarily due to the increase in revenue sharing fees and content costs.

Revenue sharing fees and content costs in the fourth quarter of 2020 increased by 25.6% to RMB1,850.2 million (US$283.6 million) from RMB1,473.3 million in the same period of 2019. This increase was primarily attributable to increased investments in the broadcasting rights for eSports tournaments and the in-house production of proprietary events, increased revenue sharing fees, which was in line with the Company’s total revenue growth, and increased investments in quality streamers in the oversea market.

Bandwidth costs in the fourth quarter of 2020 increased by 12.7% to RMB170.7 million (US$26.2 million) from RMB151.4 million in the same period of 2019, primarily due to the increase in high-quality viewing options, such as 4K high-definition video, offered on the Company’s platform, which led to higher user engagement.

Gross profit in the fourth quarter of 2020 was RMB182.2 million (US$27.9 million), compared with RMB375.2 million in the same period of 2019, which was due to the increase in revenue sharing fees and content costs as a percentage of total revenues.

Sales and marketing expenses in the fourth quarter of 2020 increased by 27.3% to RMB170.7 million (US$26.2 million) from RMB134.1 million in the same period of 2019, primarily attributable to the increase in promotional activities on key eSports tournaments and in prizes for the Company’s self-produced events.

Research and development expenses in the fourth quarter of 2020 increased by 18.6% to RMB118.9 million (US$18.2 million) from RMB100.2 million in the same period of 2019, mainly due to the Company’s application upgrades and video-related technology improvements.

General and administrative expenses in the fourth quarter of 2020 increased by 54.0% to RMB117.7 million (US$18.0 million) from RMB76.4 million in the same period of 2019, mainly due to the increase in professional service fees related to the Company’s potential merger with Huya.

Other operating loss, net in the fourth quarter of 2020 was RMB7.3 million (US$1.1 million), compared with other operating income of RMB59.7 million in the same period of 2019.

Loss from operations in the fourth quarter of 2020 was RMB232.4 million (US$35.6 million), compared with an operating income of RMB124.1 million in the same period of 2019.

Adjusted operating loss¹ in the fourth quarter of 2020, which adds back share-based compensation expenses, was RMB199.1 million (US$30.5 million), compared with an adjusted operating income of RMB141.5 million in the same period of 2019.

Income tax expenses in the fourth quarter of 2020 and 2019 were nil due to the Company’s cumulative net losses and the resulting tax loss carry forward.

Net loss in the fourth quarter of 2020 was RMB228.7 million (US$35.0 million), compared with a net income of RMB157.4 million in the same period of 2019.

Adjusted net loss in the fourth quarter of 2020, which excludes share-based compensation expenses, share of income in equity method investments, and impairment loss of investments, was RMB176.9 million (US$27.1 million), compared with an adjusted net income of RMB186.4 million in the same period of 2019, implying an adjusted net loss margin of 7.8% for the fourth quarter of 2020.

Basic and diluted net loss per ADS² in the fourth quarter of 2020 were RMB0.61 (US$0.09) and RMB0.59 (US$0.09) respectively. Adjusted basic and diluted net loss per ADS in the fourth quarter of 2020 were RMB0.45 (US$0.07) and RMB0.45 (US$0.07) respectively.

Full Year 2020 Financial Results

Total net revenues for the full year of 2020 increased by 31.8% to RMB9,601.9 million (US$1,471.6 million) from RMB7,283.2 million in the same period of 2019, primarily driven by a 33.8% year-over-year increase in live streaming revenues.

Gross profit for the full year of 2020 increased by 30.4% to RMB1,560.3 million (US$239.1 million) from RMB1,196.2 million in the same period of 2019. Gross margin in the full year of 2020 was 16.3%.

Income from operations for the full year of 2020 was RMB262.1 million (US$40.2 million), compared with a loss from operations of RMB131.7 million in the same period of 2019.

Adjusted operating income for the full year of 2020, which adds back share-based compensation expenses, increased by 154.0% to RMB404.2 million (US$62.0 million) from RMB159.1 million in the same period of 2019.

Net income for the full year of 2020 increased by 1,113.7% to RMB404.7 million (US$62.0 million) from RMB33.3 million in the same period of 2019.

Adjusted net income for the full year of 2020, which excludes share-based compensation expenses, share of loss (income) in equity method investments, and impairment loss of investments, increased by 56.3% to RMB541.6 million (US$83.0 million) from RMB346.4 million in the same period of 2019, implying an adjusted net margin of 5.6% for the full year of 2020.

Basic and diluted net income per ADS for the full year of 2020 were RMB1.52 (US$0.23) and RMB1.47 (US$0.23) respectively. Adjusted basic and diluted net income per ADS in the full year of 2020 were RMB1.95 (US$0.30) and RMB1.95 (US$0.30) respectively.

Conference Call Information

The Company will hold a conference call on Tuesday, March 23, 2021, at 8:00 am Eastern Time (or 8:00 pm Beijing Time on the same day) to discuss the financial results. Listeners may access the call by dialing the following numbers:

The replay will be accessible through March 30, 2021, by dialing the following numbers:

A live and archived webcast of the conference call will also be available at the Company’s investor relations website at http://ir.douyu.com/.

About DouYu International Holdings Limited

Headquartered in Wuhan, China, DouYu International Holdings Limited (Nasdaq: DOYU) is a leading game-centric live streaming platform in China and a pioneer in the eSports value chain. DouYu operates its platform on both PC and mobile apps, through which users can enjoy immersive and interactive games and entertainment live streaming. DouYu’s platform brings together a deep pool of top live streamers. By providing a sustainable streamer development system built on advanced technology infrastructure and capabilities, DouYu helps ensure a consistent supply of quality content. Through collaborations with a variety of participants across the eSports value chain, the Company has gained coveted access to a wide variety of premium eSports content, which further attracts viewers and enhances user experience. For more information, please see http://ir.douyu.com/.

Use of Non-GAAP Financial Measures

Adjusted operating income (loss) is calculated as operating income (loss) adjusted for share-based compensation expenses. Adjusted net income is calculated as net loss adjusted for share-based compensation expenses, share of income (loss) in equity method investments, gain on disposal of investment or subsidiaries and impairment loss on investments. Adjusted net income attributable to DouYu is calculated as net income attributable to DouYu adjusted for share-based compensation expenses, share of income (loss) in equity method investments, gain on disposal of investment or subsidiaries and impairment loss of investments. Adjusted basic and diluted net income per ordinary share is non-GAAP net income attributable to ordinary shareholders divided by weighted average number of ordinary shares used in the calculation of non-GAAP basic and diluted net income per ordinary share. The Company adjusted the impact of (i) share-based compensation expenses, (ii) share of income (loss) in equity method investments, (iii) gain on disposal of investment or subsidiaries and (iv) impairment loss of investments to understand and evaluate the Company’s core operating performance. The non-GAAP financial measures are presented to enhance investors’ overall understanding of the Company’s financial performance and should not be considered a substitute for, or superior to, the financial information prepared and presented in accordance with U.S. GAAP. Investors are encouraged to review the reconciliation of the historical non-GAAP financial measures to its most directly comparable GAAP financial measures. As non-GAAP financial measures have material limitations as analytical metrics and may not be calculated in the same manner by all companies, they may not be comparable to other similarly titled measures used by other companies. In light of the foregoing limitations, you should not consider non-GAAP financial measures as a substitute for, or superior to, such metrics in accordance with U.S. GAAP.

For more information on these non-GAAP financial measures, please see the table captioned “Reconciliations of Non-GAAP Results” near the end of this release.

Exchange Rate Information

This announcement contains translations of RMB amounts into U.S. dollars at a specified rate solely for the convenience of the reader. Unless otherwise noted, all translations from RMB to U.S. dollars are made at a rate of RMB6.5250 to US$1.00, the noon buying rate in effect on December 31, 2020, in the H.10 statistical release of the Federal Reserve Board. The Company makes no representation that the RMB amounts could have been, or could be, converted, realized or settled in U.S. dollars at that rate on December 31, 2020, or at any other rate.

Safe Harbor Statement

This press release contains forward-looking statements. These statements are made under the “safe harbor” provisions of the U.S. Private Securities Litigation Reform Act of 1995. Statements that are not historical facts, including statements about the Company’s beliefs and expectations, are forward-looking statements. Forward-looking statements involve inherent risks and uncertainties, and a number of factors could cause actual results to differ materially from those contained in any forward-looking statement. In some cases, forward-looking statements can be identified by words or phrases such as “may,” “will,” “expect,” “anticipate,” “target,” “aim,” “estimate,” “intend,” “plan,” “believe,” “potential,” “continue,” “is/are likely to” or other similar expressions. Further information regarding these and other risks, uncertainties or factors is included in the Company’s filings with the Securities Exchange Commission. All information provided in this press release is as of the date of this press release, and the Company does not undertake any duty to update such information, except as required under applicable law.

In addition to factors previously disclosed in Huya and DouYu’s documents filed with the SEC, the following factors, among others, could cause actual results to differ materially from forward-looking statements and information or historical performance: the occurrence of any event, change or other circumstances that could give rise to the right of one or both of Huya and DouYu to terminate the definitive merger agreement between Huya and DouYu; the outcome of any legal proceedings that may be instituted against Huya, DouYu or their respective shareholders or directors; the ability to obtain regulatory approvals and meet other closing conditions to the merger, including the risk that regulatory approvals required for the merger are not obtained or are obtained subject to conditions that are not anticipated or that are material and adverse to Huya’s or DouYu’s business; a delay in closing the merger; the ability to obtain approval by DouYu’s shareholders on the expected terms and schedule; business disruptions from the proposed merger that will harm Huya’s or DouYu’s business, including current plans and operations; potential adverse reactions or changes to business relationships resulting from the announcement or completion of the merger; certain restrictions during the pendency of the merger that may impact Huya’s or DouYu’s ability to pursue certain business opportunities or strategic transactions; the ability of Huya or DouYu to retain and hire key personnel; uncertainty as to the long-term value of the Class A ordinary shares of Huya following the merger; the continued availability of capital and financing following the merger; the business, economic and political conditions in the markets in which Huya and DouYu operate; changes in Huya’s or DouYu’s anticipated revenue and income; changes in DouYu’s operating or other expenses; the degree to which Huya or DouYu encounters competition; and general political, economic and market conditions.

Investor Relations Contact

Mao Mao

DouYu International Holdings Limited

Email: ir@douyu.tv

Phone: +1 (646) 224-6934

Xinran Rao

ICR, Inc.

Email: DouYu.IR@icrinc.com

Phone: +1 (646) 224-6934

Media Relations Contact

Iris Ding

DouYu International Holdings Limited

Email: pr_douyu@douyu.tv

Phone: +1 (646) 308-1475

Edmond Lococo

ICR, Inc.

Email: DouYu.PR@icrinc.com

Phone: +1 (646) 308-1475

UNAUDITED CONDENSED CONSOLIDATED BALANCE SHEETS

(All amounts in thousands, except share, ADS, per share and per ADS data)

UNAUDITED CONDENSED CONSOLIDATED BALANCE SHEETS (CONTINUED)

(All amounts in thousands, except share, ADS, per share and per ADS data)

UNAUDITED CONDENSED CONSOLIDATED STATEMENTS OF INCOME (LOSS)

(All amounts in thousands, except share, ADS, per share and per ADS data)

RECONCILIATIONS OF GAAP AND NON-GAAP RESULTS

(All amounts in thousands, except share, ADS, per share and per ADS data)